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This 15 page paper addresses this major issue. Recent studies have focused on the ability to monitor expression of genes in a bacterium, especially in those bacteria with genome that have been sequenced. Escherichia coli (E. coli) is a bacterium that has recently been sequenced, and this study attempts to determine the elements that change the expression, including upregulation and down regulations of the genome of E. coli. This study provides a view of the impacts of environmental conditions (aerobic, anaerobic, microaerobic or the presence of potassium nitrate) on the expression of E. coli-12. Bibliography lists 10 sources.
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15 pages (~225 words per page)
File: MH11_MHEcoli2.rtf
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has recently been sequenced, and this study attempts to determine the elements that change the expression, including upregulation and down regulations of the genome of E. coli. This study
provides a view of the impacts of environmental conditions (aerobic, anaerobic, microaerobic or the presence of potassium nitrate) on the expression of E. coli-12. This study shows what factors
determine how genes are affected by environmental differences and how this influences assessments of expression in E. coli. KEYWORDS Aerobic conditions Anaerobic conditions Bacterial genome sequences Biochemical pathways
Bioinformatic inferences Escherichia coli Gene expression KNO (potassium nitrate) Microaerobic conditions Pathogenesis INTRODUCTION In recent years, a number of studies of strains of Escherichia coli have occurred, as a result
of the assessment of the bacterium as a substantial threat to human health (Weitzman, 2001). The non-pathogenic laboratory E. coli strain K-12 has been at the center of studies,
both as a comparison with pathogenic strains, including 0157:H7, and as a means of determining the development of new genes (Weitzman, 2001). IN a study in the January 25
Nature, Perna et al made a comparison of the genome of non-pathogenic laboratory E. coli strain K-12 with E. coli strain 0157:H7, only to discover that there were 1,387 new
genes in this second strain (Weitzman, 2001). Researchers, then, have recognized the importance of assessing the non-pathogenic K-12 strain and assessing possible influences on gene expression. Initially,
the complete genome sequence of Escherichia coli K-12 was conducted by Frederick Blattner et al and published in a 1997 article in Science. The Blattner group took over 15
years to complete their project from its conception, with the goal of a complete genome sequencing of Escherichia coli K-12 (M1655 strain). This project not only resulted in the
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