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Measuring the Cytotoxicity of Chemicals Using the LC50 Test

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This is a 12 page paper that provides an overview of measuring cytotoxicity. An experimental design measures the LC50 of ammonium hydroxide and sodium lauryl sulfate. Bibliography lists 20 sources.

Page Count:

12 pages (~225 words per page)

File: KW60_KFsci028.doc

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Unformatted sample text from the term paper:

lowering costs and increasing profit margins, than on the safe consumption of the products in question. For this reason, governments around the world have introduced legislation to regulate the practices that businesses can undertake to ensure that the resulting products are safe to the humans or animals that will eventually consume them. In order to achieve this purpose, scientists are employed to analyze the relative toxicity of the materials and byproducts that are used in production facilities. Of course, banning all materials that merely qualify as "toxic" may be overzealous, as there are millions of chemicals that are toxic to human and animal organisms when consumed in abundance. In order to create an objective metric for determining whether materials can be safety be used or not, scientists have developed the standard known as "LC50", or "lethal concentration 50%". This paper will document an experiment intended to explore the measurement of LC50 in practice by analyzing the lethal concentrations of two materials common in everyday household products: ammonium hydroxide and sodium lauryl sulfate. LC50 refers specifically to the concentration of a given material that will kill 50% of exposed organisms in a given population which is exposed to said material (Newman, 1995). For instance, a 10% dilution of a material might be relatively safe when exposed to laboratory test animals, but a 20% dilution of the same material might be sufficient to cause the death of half of those animals; in that cause, the LC50 of the material in question would be 20%. Materials with a sufficiently high LC50 are assume to be cytotoxic to humans as well as test animals, and as such are classified as being highly toxic, and are generally not allowed in manufacturing processes (Zhang et al., 2007). Such studies have a great deal of relevance to ...

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