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Essay / Research Paper Abstract
This 12 page paper examines the use of Immunological Techniques, specifically ELISAs and RIAs. The paper starts by considering the detection of the antibody, the antiserum or the antibody used to detect the antigen, the way that the bound antibody and antigen can be separated from the unbound reactants if there is the use of a heterogeneous assay and the detection method. The bibliography cites 12 sources.
Page Count:
12 pages (~225 words per page)
File: TS14_TEimmtech.rtf
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Unformatted sample text from the term paper:
pathogenic micro-organisms such as bacteria, viruses and parasites (Crowther, 1995). The immune system, operates by recognising and then trying to eradicate the pathogens. There are two methods by which the
immune system operates the use of the innate system and the adaptive system, both of these methods respond to the presence of foreign substances (Crowther, 1995). The first stage is
with the use of the innate immune system where the majority of infectious agents are check prior to establishing an infection, where these are overcome the adaptive system will be
activated. The adaptive system will create a specific reaction to the pathogen which is then remembers for future infections. Immunological techniques usually look to the testing the antigens in order
to assess the residence that either is or in not present (Crowther, 1995). The development of Immunoassays was pioneered Yalow and Berson in 1959 who developed the radioimmunoassay (RIA) that
could be used commercially. The test was specifically for human insulin (Crowther, 1995). The use of immunoassay is to detect the presence of the antigen, and although this test was
developed for human use the same technology developed and expanded into the other areas such as for use in equines. The development of immunoassay techniques is the now the
most common tool used to detect antibodies and test the immune system. RIA and enzyme-linked immuno sorbent assay (ELISA) are both immunoassay techniques so an overview of these increases understanding
of both and other immunoassay techniques as they have the same four components. The first component in the detection of the antibody, the second component is the antiserum or
the antibody used to detect the antigen, the third component is the way that the bound antibody and antigen can be separated from the unbound reactants if there is the
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